Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit.
Identifieur interne : 000808 ( 2020/Analysis ); précédent : 000807; suivant : 000809Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit.
Auteurs : Arun K. Nalla [États-Unis] ; Amanda M. Casto [États-Unis] ; Meei-Li W. Huang [États-Unis] ; Garrett A. Perchetti [États-Unis] ; Reigran Sampoleo [États-Unis] ; Lasata Shrestha [États-Unis] ; Yulun Wei [États-Unis] ; Haiying Zhu [États-Unis] ; Keith R. Jerome [États-Unis] ; Alexander L. Greninger [États-Unis]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2020.
Abstract
Nearly 400,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Washington. Given the rapid increase in the number of cases in many localities, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis. In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we evaluated assays using seven different primer/probe sets and one assay kit. We found that the most sensitive assays were those that used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25 (3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set developed by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020, https://www.cdc.gov/coronavirus/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf). All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide valuable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.
DOI: 10.1128/JCM.00557-20
PubMed: 32269100
Affiliations:
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Nalla</name><affiliation wicri:level="2"><nlm:affiliation>Department of Laboratory Medicine, University of Washington, Seattle, WA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Laboratory Medicine, University of Washington, Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Casto, Amanda M" sort="Casto, Amanda M" uniqKey="Casto A" first="Amanda M" last="Casto">Amanda M. Casto</name><affiliation wicri:level="2"><nlm:affiliation>Department of Medicine, University of Washington, Seattle, WA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Medicine, University of Washington, Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Huang, Meei Li W" sort="Huang, Meei Li W" uniqKey="Huang M" first="Meei-Li W" last="Huang">Meei-Li W. 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Jerome</name><affiliation wicri:level="1"><nlm:affiliation>Department of Laboratory Medicine, University of Washington, Seattle, WA kjerome@fredhutch.org agrening@uw.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country></affiliation></author><author><name sortKey="Greninger, Alexander L" sort="Greninger, Alexander L" uniqKey="Greninger A" first="Alexander L" last="Greninger">Alexander L. Greninger</name><affiliation wicri:level="1"><nlm:affiliation>Department of Laboratory Medicine, University of Washington, Seattle, WA kjerome@fredhutch.org agrening@uw.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nearly 400,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Washington. Given the rapid increase in the number of cases in many localities, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis. In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we evaluated assays using seven different primer/probe sets and one assay kit. We found that the most sensitive assays were those that used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25 (3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set developed by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020, https://www.cdc.gov/coronavirus/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf). All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide valuable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Nalla, Arun K" sort="Nalla, Arun K" uniqKey="Nalla A" first="Arun K" last="Nalla">Arun K. Nalla</name></region><name sortKey="Casto, Amanda M" sort="Casto, Amanda M" uniqKey="Casto A" first="Amanda M" last="Casto">Amanda M. Casto</name><name sortKey="Greninger, Alexander L" sort="Greninger, Alexander L" uniqKey="Greninger A" first="Alexander L" last="Greninger">Alexander L. Greninger</name><name sortKey="Huang, Meei Li W" sort="Huang, Meei Li W" uniqKey="Huang M" first="Meei-Li W" last="Huang">Meei-Li W. Huang</name><name sortKey="Jerome, Keith R" sort="Jerome, Keith R" uniqKey="Jerome K" first="Keith R" last="Jerome">Keith R. Jerome</name><name sortKey="Perchetti, Garrett A" sort="Perchetti, Garrett A" uniqKey="Perchetti G" first="Garrett A" last="Perchetti">Garrett A. Perchetti</name><name sortKey="Sampoleo, Reigran" sort="Sampoleo, Reigran" uniqKey="Sampoleo R" first="Reigran" last="Sampoleo">Reigran Sampoleo</name><name sortKey="Shrestha, Lasata" sort="Shrestha, Lasata" uniqKey="Shrestha L" first="Lasata" last="Shrestha">Lasata Shrestha</name><name sortKey="Wei, Yulun" sort="Wei, Yulun" uniqKey="Wei Y" first="Yulun" last="Wei">Yulun Wei</name><name sortKey="Zhu, Haiying" sort="Zhu, Haiying" uniqKey="Zhu H" first="Haiying" last="Zhu">Haiying Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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